I am trying to do some DNA extraction for my professor, and I make a lysis buffer with proteinase K. I did not have the time to continue the procedure so the hair and the buffer have been sitting together at room temperature for about 12 days. My professor stated it would be "fine" to continue on with my DNA isolation procedure, but I have my doubts.
Should I continue my DNA extraction( vortexing, boiling, centrifugation and collection of supernatant, storage at -20 degree Celsius) or would this be pointless at this time? My goal is to run PCR and then a gel.
EDIT:
Here is the composition of the lysis buffer:
In 20 ml of Lysis buffer:
final concentrations are 0.1M TAE, 0.5M NaCl, 0.2% SDS.
To 800ul of Lysis buffer I added 5ul of proteinase K at a concentration of 250ug/ml.
(Proteinase K was made via: 0.0025g up to 10ml of TAE buffer( 1M))
This was in a 1.5ml centrifuge tube that has been now sitting for 12 days.