Mutations in which of the following regions upstream of the RNA coding sequence are LEAST likely to affect the transcription of a gene?
A. -10 consensus sequence within the promoter region
B. -35 consensus sequence within the promoter region
C. the region between -10 and -35 consensus sequences
D. the upstream elements at -40 to -60 position
E. Any mutation on the upstream of a gene will definitely have deleterious effect on gene transcription.