Which of the following would NOT be true of cDNA produced using human brain tissue as the starting material?
a. It was produced from mRNA using reverse transcriptase.
b. It could be used to create a complete genomic library.
c. It could be amplified by the polymerase chain reaction.
d. It could be used as a probe to detect genes expressed in the brain.
e. It lacks the introns of the human genes.

cDNA or complementary DNA is synthesized from the single-stranded RNA. The reaction is catalyzed by the enzyme reverse transcriptase. It is used to clone eukaryotic genes in the monerans.

The correct answer is:

Option B. It could be used to create a complete genomic library.

The use of cDNA can be explained as:

1. cDNA is synthesized from the mRNA and the reaction is catalyzed by the enzyme reverse transcriptase.

2. The complementary DNA can be amplified using polymerase chain reaction, as it is widely used to clone the eukaryotic genes in the prokaryotes.

3. cDNA is expressed in the prokaryotes because prokaryotes do not consist of introns.

4. cDNA is a transcriptome of a particular organism, whereas the genome of an organism can only be expressed by genomic DNA.

Thus, the correct answer is that it could be used to create a complete genomic library.

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