The correct answer is option D ("The uncertain nature of human relations").
In this excerpt from Robert Frost's "Mending Wall" we can distinguish a thematical focus on the many difficulties that people face when trying to connect to each other. The author observes how we tend to lift up walls around ourselves coming from a place of fear and other unhealthy mindsets and behaviors that keep us farther away from each other that we'd like many times. Another important point he brings up is that these barriers we create are often a product of cultural segregation, and we are prone to keep them up preventing threats (often imagined) towards our identity.
Hope this helps!