Select the statement that is true for why is a blank measured before the sample. Select one: a. A blank is measured to remove the signal caused by the cuvette walls and solvent. b. A blank is measured to calibrate the spectrophotometer's light source. c. There is no reason to measure a blank before the sample. d. A blank is measured to clean the spectrophotometer.

Spectrophotometer is an instrument for measuring the absorbance or transmittance of the liquid in terms of wave function. The blank solutions is used for calibration of spectrophotometer while the cuvette is the container where the liquid sample is taken in the spectrophotometer. The absorbance of blank solution is known as zero hence the name blank solution. Normally, a blank is is measured to eliminate signal caused by cuvette walls on solvent and results into the formation of solute peaks in the spectra.

shrutiagrawal1798 shrutiagrawal1798 · Answer 2 · 2022-02-23T08:05:49+01:00

Spectroscopy is the measurement of the absorbance by the sample molecules. The blank is used to remove the cuvette and solvent signals.

What is a blank in a spectrometer?

Spectroscopy is an analytical technique that allows the light radiation to pass through the sample, and measure the absorbance of the sample molecules.

The measurement of sample absorbance is done after the blank measurement. The blank is the solution without the sample molecules.

The measurement of blank signals helps to remove the absorbance for the cuvette and the solvent molecules, thereby resulting in the complete and accurate sample absorbance.

Thus, blank measurement is performed in the spectrometer to remove the signals from the cuvette and the solvent. Thus, option A is correct.

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