Hydropathy plot analysis of a protein of interest reveals a single, prominent hydrophobic peak. However, it is later discovered that this protein is soluble and not membrane associated. What may have caused the hydropathy plot to be misleading?
The hydropathy plot will be misleading when there is a stretch of hydrophobic amino acids like the ones seen in transmembrane helices due to the alpha helix that goes through the protein hydrophobic core.
