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What are the features of pComb8 vector?

VPT4011 is nearly identical with pComb3 but contains the phage pVIII fusion protein for multi-valent expression along the sides of the phage via fusion with pVIII.

https://www.creative-biogene.com/pComb8-vector-VPT4011-1232907-73.html

Respuesta :

Features of pComb8 vector

Explanation:

The Comb8 vector is a type of a phage display plasmid or phagemid.

A phagemid is basically created by fusing the target protein to the phage particle’s surface utilizing in vitro screening techniques

Phagemids are used for studying and  screening protein-peptides

The pComb8 vector phagemid:

  • Belongs to category VPT 4011 which is similar to Comb3 vector except that the sequence of gene III is replaced by gene VIII sequence
  • The Comb8 clones the heavy chain sequence into the Xhol/Spel restriction sites and the light chain sequences into the Sacl/Xbal restriction sites for Fab display.
  • Protein VIII is naturally copied on to pComb8 for multivalent display
  • Contains enough soluble protein

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