Answer: The correct order is
D. Isolate donor cells from egg donor and germ cell donor
C. Remove and discard the nucleus from the egg cell
E. Transfer the somatic cell nucleus into the egg cell
A. Stimulate cell division
F. Implant embryo into a surrogate mother
B. Deliver baby
Explanation: SCNT (somatic cell nuclear transfer) is a technique used to transfer the nucleus of a somatic (body) cell to the cytoplasm of an enucleated egg (an egg whose nucleus has been removed).
The steps involved in SCNT are:
1) Preparation and extraction of the somatic cell: This step involves preparation and extraction of somatic (body) cell from any part of the body but preferably the skin.
2). Enucleation: This step involves the removal of the egg's nucleus.
3). Nuclear transfer: This involves the transfer of the somatic cell nucleus into the enucleated egg.
4). Egg incubation: It involves the incubation of the egg in a medium that stimulates cell division and conducive for embryonic development. Once the somatic cell nucleus is transfer into the egg, the cytoplasmic factors of the egg reprogramme somatic cell nucleus to become a nucleus of a zygote (fertilized egg). The zygote then develops to the blastocyst stage.
5). Embryogenesis: This involves the implantation of the blastocyst into the surrogate mother who carries the developing embryo.
6). The surrogate mother carries the developing embryo like a normal pregnancy and delivers the baby when it is due.
