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"An orange compound was added to the top of a chromatography column. Solvent was added immediately, with the result that the entire volume of solvent in the solvent reservoir turned orange. No separation could be obtained from the chromatography experiment. What went wrong?

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Answer:The result indicates that the polar component of the mixture fail to separate. In a case like this a third liquid like acid, base or complexing agent is often added to retain more water in the organic solvent.

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Seems to me that the sample had not been properly adsorbed, did not establish a true equilibrium, and therefore washed through the column rather that separated. The sample should be allowed to stand a short time before elution rather than putting the solvent in immediately.

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