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Isoelectric point. The isoelectric point (pI) is the pH at which a molecule has no net charge. The amino acid glycine has two ionizable groups: (1) a carboxylic acid group with a pKa of 2.72 and (2) an amino group with a pKa of 9.60. Calculate the pI of glycine

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Answer:

pI = 6.16

Explanation:

The pI is given by the average of the pKas that are involved. In this case,

Pka of carboxylic acid was given as 2.72 and that of the Amino group was given as 9.60. the average would then be ½(2.72+9.60)

= 6.16

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