"In a translation experiment using a fungus culture, radiolabeled" amino acids were added to the culture, allowing the researchers to measure the growth of a single polypeptide chain by measuring counts per minute (CPM). As the chain grew, the CPM increased. After a certain amount of time, CHX was added to the mixture, and the experiment continued. After an additional amount of time, the CHX was removed from the culture medium. Which of the following graphs best predicts the data collected during the experiment?

Question

26-02-2020
Chemistry

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"In a translation experiment using a fungus culture, radiolabeled" amino acids were added to the culture, allowing the researchers to measure the growth of a single polypeptide chain by measuring counts per minute (CPM). As the chain grew, the CPM increased. After a certain amount of time, CHX was added to the mixture, and the experiment continued. After an additional amount of time, the CHX was removed from the culture medium. Which of the following graphs best predicts the data collected during the experiment?

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KerryM KerryM · Answer 1 · 2020-03-03T17:50:15+01:00

KerryM KerryM

03-03-2020

Question: Graphs found from another source attached

Answer:

Graph D (labelled left of X axis)

Explanation:

CHX or cyclohexamide is a drug that inhibits protein synthesis and therefore translation.

The growth of a single polypeptide chain can only occur when protein synthesis is occurring via translation. Therefore, when CHX is added to the culture, it will stop, and when it is removed, will start again.

Therefore, the graph would show a polypeptide chain growing, ceasing growth upon the addition of CHX, and then increasing growth once it is removed, which is shown in graph D

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