Why is biochemical purification of one gene away from the other genes in a cell more challenging than biochemical purification of one protein away from the other proteins in a cell?

a)A cell has many more genes that it has proteins.
b) DNA is very unstable and is likely to be broken down during the purification process.
c) Proteins are larger molecules than genes.
d)The chemical nature of all genes is very similar; the chemical nature of various proteins can be very different.

The isolation of proteins requires protein separation techniques and identification which can identify individual proteins because of their distinct differences as well as different charges but in the case of genes, their chemical nature comprising of DNA are the same.

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luisvaschetto luisvaschetto · Answer 2 · 2021-11-23T11:29:43+01:00

The purification of one gene from the other genes is more challenging than the biochemical purification proteins because the chemical nature of all genes is very similar (Option d).

DNA is a double helix molecule composed of two long chains of nucleotides linked by hydrogen bonds.

In DNA, there are four types of nucleotides, each containing one different type of nitrogenous base (i.e., Adenine, Guanine, Cytosine, and Thymine)

Genes are very similar chains of nucleotides, which are always composed of specific single-strand fragments of the DNA molecule.

Conversely, proteins have specific tridimensional (3D) structures and these macromolecules are composed of 20 different types of amino acids.

In conclusion, the purification of one gene from the other genes is more challenging than the biochemical purification proteins because the chemical nature of all genes is very similar (Option d).

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