Answer:
Plasmids
Explanation:
The cellular components are important in the production of the protein luciferase. The protein is produced by the reaction in the silk worms and the fireflies. In addition, the protein in addition, the correct sequencing is essential in the coding of the protein. The concentration of the protein in the cytoplasm makes the control of the production of the protein feasible under feasible conditions.
luciferase protein from mRNAs in Photinus pyralis luciferase cDNA is a fragment of about 1.6 kb that encodes a polypeptide of 550 amino acid residues.
Luciferase has as substrate a carboxylic acid complex called luciferin. Luciferases are bifunctional enzymes that catalyze the oxidation of luciferin by oxygen, activated by MgATP.
In the first step of enzymatic catalysis, luciferase acts as adenyl transferase, adenylating luciferin from ATP and releasing pyrophosphate.
The luciferase then acts as an oxygenase, removing the proton from the alpha carbon to the carbonyl, making it susceptible to attack by molecular oxygen, with the production of the dioxethanone intermediate, whose cleavage produces carbon dioxide and excited oxyluciferin.
With this information, we can conclude that the cellular component and the location of the component responsible for the production of luciferase protein from mRNAs transcribed in T lymphocytes containing plasmids.
was cloned, sequenced and the primary structure of the protein was deduced. Photinus pyralis luciferase cDNA is a fragment of about 1.6 kb that encodes a polypeptide of 550 amino acid residues.
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