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The primers you used in lab amplified 145 base pairs that were adjacent, but not included in the repeat. If you used a different primer that only amplified 130 base pairs adjacent to a 14 base pair repeat, and you had 6 repeats in your product, what was the size of your final PCR product?

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adebayodeborah8

Answer:

The answer is 214.

Explanation:

This is because 130 base pair is adjacent to 14 base pair repeat , and you have 6 repeats. So 14 multiplied by 6 repeats is 84. If you add this to your primer amplicon, you will get the size of final PCR product i.e.. 214.

batolisis

The size of your final PCR product will be ; 214

Given that the new primer used amplifies 130 base pairs that are adjacent to

a) 14 base pair  and

b) Has 6 repeats

Determine the size of the final PCR product

( number of base pair * number of repeats ) + Initial/primer amplicon value

= ( 14 * 6 ) + 130

= 84 + 130

= 214

Hence we can conclude that the size of the final PCR product is 214

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