Answer:
Explanation:
From the given information ;the objective is to determine how the instructor made the 0.25L elution buffer
0.25 L elution buffer = 250 mL elution butter
The breaking buffer that we use this week contains
10mM Tris = 0.01 M
150mM NaCl = 0.15 M
300mM imidazole. = 0.3 M
The stock concentration of Tris in 1M
Therefore ; by using the formula: [tex]M_1V_1 = M_2 V_2[/tex]; we can determine the volume in the preparation; so;
[tex]1*V_1 = 0.0 1 \ M * 250 \ mL[/tex]
[tex]V_1 = \dfrac{0.0 1 \ M * 250 \ mL}{1 }[/tex]
[tex]V_1 = 2.5 \ mL[/tex]
In NaCl, The amount of stock concentration is 5 M
so; using the same formula; we have:
[tex]5*V_1 = 0.15 \ M * 250 \ mL[/tex]
[tex]V_1 = \dfrac{0. 15 \ M * 250 \ mL}{5 }[/tex]
[tex]V_1 = 7.5 \ mL[/tex]
From Imidazole ; the amount of stock concentration is
[tex]1*V_1 = 0.3 \ M * 250 \ mL[/tex]
[tex]V_1 = \dfrac{0. 3 \ M * 250 \ mL}{1 }[/tex]
[tex]V_1 = 75 \ mL[/tex]
Thus; we can have a table as shown as :
Stock concentration volume to be added Final concentration
1 M of Tris 2.5 mL 10 mM
5 M of NaCl 7.5 mL 150 mM
1 M of Imidazole 75 mL 300 mM
In conclusion. the addition of all the volume make up the 250 mL elution buffer that is equivalent to 0.25 L.
