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Researchers grew populations of identical Escherichia coli (E. coli) bacteria in a growth medium that contained a low concentration of glucose and a high concentration of citrate, a substance that is not typically consumed by E. coli. For thousands of generations, the bacteria used only glucose as an energy source and grew relatively slowly and to a low density because of the low concentration of glucose. After about 30,000 generations, one population emerged that began to rapidly grow to a much higher density. The researchers hypothesized that the bacteria evolved the ability to use citrate as an energy source and referred to them as Cit . To test the hypothesis, the researchers grew separate populations of the Cit bacteria and bacteria from the original population (Cit- ) in a growth medium that contained only citrate. Describe one outcome that would demonstrate that a given population has evolved.

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Answer:

The colony of bacteria is called cit positive, or cit in case these advance with colonization in media with citrate, since if they grow in number and proliferate, it means that they use citrate as a metabolite or food resource for their metabolism.

Explanation:

In case these are negative cit, the profileration would have to be null or see that the colony stagnant its growth, that is to say that they did not increase in number or even decrease their number layers and they did not replicate in media with citrate.

The colony of bacteria is named cit positive, or cit just in case these advance with colonization in media with citrate.

Since if they grow in number and proliferate, it means they use citrate as a metabolite or food resource for metabolism.

In case these are negative cit, the proliferation would have to be null or see that the colony stagnant its growth, that is to say, that they did not increase in number or maybe decrease their number layers and that they didn't replicate in media with citrate.

What about E.coli?

E. coli, a species of bacteria that lives harmlessly in every person’s gut by the billions. A typical E. coli contains about 4,000 genes (we have about 20,000). Feeding on sugar, the microbe grows till it's able to split in two.

It makes two copies of its genome, nearly always managing to supply perfect copies of the first. The single microbe splits in two, and every new E. coli receives one of the identical genomes. These two bacteria are, in other words, clones.

Therefore, E.coli grows in presence of citrate that evolved in the population of 30,000 generations.

Learn more about E.coli here:

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