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I need help. What is a cloning vector? How are cloning vectors used? Give three examples of cloning vectors, indicating which ones are better for small fragments of DNA and which are better for large fragments.

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martinikennedy2

Answer:

1. A cloning vector is a small piece of DNA that can be stably maintained in an organism, and into which a foreign DNA fragment can be inserted for cloning purposes.

2. Cloning vectors are plasmids that are used primarily to propagate DNA. They replicate in E. coli to high copy numbers and contain a multiple cloning site

3a. Examples of cloning vectors are plasmids, cosmids, bacterial artificial chromosomes (BACs), and yeast artificial chromosomes (YACs).

3b. plasmids- small, BACs- large, YACs- large, cosmids- large

Explanation:

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