Answer:
1.) Subject = 18 Aquariums each with mussel
2.) Factors(levels) = temperature(3 levels) and salinity (2 levels)
3.) Treatment = High salinity high temperature ; High salinity low temperature ; High Salinity medium temperature ; Low salinity Low temperature ; Low salinity, High temperature ; Low salinity Medium temperature.
4.) Response variable = size of mussel produced
5.) Random assignment will help prevent the introduction of bias and confounding variable.
6.) For replication purpose, Each of the six treatment could be assigned to 3 tanks, so 3 aquarium for one treatment.
7.) By doing such we might be a possible location based effect which might might creep into our research. Hence, causing a spurious relationship.
8.) A control experiment whereby no salt is added will cause the mussel to die as they require salt to survive.
Step-by-step explanation:
Answers are Explanatory.