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The oxidase test is another biochemical test used to distinguish aerobic versus anaerobic metabolism in microbes. Research the oxidase test and describe how it is performed. Include examples of oxidase-positive and oxidase-negative microbes in your answer.

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lenny2006
The Bacillus genus is usually negative in regards for the Oxidase test.
ajinems

Oxidase test is a biochemical test that is used to identify organisms that produce the enzyme  oxidase.

Oxidase test is used to distinguish aerobic from anaerobic metabolism in microbes.

Aerobic metabolism is the use of oxygen to generate energy from a food source while anaerobic metabolism is the generation of energy with the absence of oxygen.

The procedure of oxidase test includes:

  • Place a piece of filter paper in a clean petri dish and add 2 or 3 drops of freshly prepared oxidase reagent.
  • Using a piece of stick or glass rod (not anoxidized wire loop), remove a colony of the test organism and smear it on the filter paper.
  • Look for the development of a blue-purplecolour within a few seconds as shown in colour

Blue purple colour signifies oxidase positive organisms which includes Pseudomonas aeruginosa

Colourless signifies oxidase negative organisms which includes Escherichia coli

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https://brainly.com/question/4680704

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