In order to observe a specimen under 4X objective lens, the following procedures must be observed:
- Set up the microscope ensuring that the lens have been adequately cleaned and the light source is working
- Mount the object on a slide and place the slide on the stage of a clean microscope.
- Switch the revolving nose piece to ensure that the 4X objective is in position
- Switch on the light source
- Bring the object into focus by using coarse and fine adjustment knobs.
- Ensure that the image of the object is centered on the stage.
In order to observe the same specimen under the 100X objective:
- Switch the revolving nose piece to fix the 100x objective in place.
- Only use the fine adjustment knob to bring the image into focus
- If the image will not resolve properly, a few drops of immersion oil may be applied to the slide to improve resolution.
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