Vectors may be plasmids. An example of one of several useful features of commercially prepared cloning vectors is MULTIPLE CLONING SITES.
The pUC18 vector is a widely used standardized cloning vector for replication in Escherichia coli.
A multiple cloning site can be defined as a short DNA fragment observed in genetically engineered plasmids.
These DNA fragments (multiple cloning sites) contain twenty (20) or more sites where restriction enzymes can cut in order to generate recombinant DNA molecules.
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