Unicellular organism are one celled but multicellular organisms are made up of many cells.
As the name suggests, unicellular organisms are made up of only one cell and this single cell carries out all the essential functions that are needed for the survival of the organism like respiration, photosynthesis etc.
But in multicellular organization, the organisms are made of more than one cells and cells are grouped according to their functions. Each group is specialized for specific functions. Even in higher multicellular organisms the group of cells form specific types of tissues and further they form organs and organ systems which function more efficiently.
Thus, this is the difference.
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