The substrate and enzyme would bind and react before all temperatures could be tested.
Before all temperatures could be tested, the enzyme would stop functioning after a short while.
The enzyme and the substrate would bind and the reaction would breakdown hydrogen peroxide into hydrogen and oxygen. Once the majority of the substrate was consumed, the reaction would not proceed even if the enzyme was at the optimal temperature.
Specific substrate binding is a property of enzymes. For example, the enzyme catalase exclusively binds to hydrogen peroxide as a substrate.
The enzyme forms a complex with the substrate when it attaches to its active site, breaking down the substrate into products.
I understand the question you are looking for is this:
When testing the temperature of catalase, what would happen if you changed the temperature using the same tube with the same catalase and hydrogen oxide mixture?
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