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Vibration loading on adipose stem cell:
Mechanical stimulation has a significant impact on bone cell and precursor metabolism. BM or osteogenic medium (OM), which is BM augmented with 250 M ascorbic acid 2-phosphate, 5 nM dexamethasone, and 10 mM-glycerophosphate, were both used to cultivate human ASCs. The effects on attachment, viability, proliferation, alkaline phosphatase activity, and mineralization were examined after hASCs were stimulated with high frequency (50 and 100 Hz) and high magnitude (3 g) vibration for three hours each day for two weeks using a specially designed vibration loading device.
Without vibration, control cells were grown similarly. ASDWe hypothesized that vibration loading would stimulate bone-forming cell differentiation while inhibiting fat tissue differentiation in human adipose stem cells (hASCs). We created a vibration-loading device that delivers 3g of peak acceleration to cells cultured in well plates at frequencies of 50 and 100 Hz.
Vibration-loading device and principleof operation
controllable vibration-loading device, designed andbuilt at the Biomedical Engineering Department. A block diagram of the complete system is shown. An electromagnetic linear motionengine composed of a sound-producing Lab 12 subwoo-fer (Eminence Speaker LLC, KY, USA) was employedto produce the vibration loading. The unit was drivenby a microcontroller-based signal source that was pro-vided with a direct accelerometer feedback. TheADXL321 accelerometer. Analogue Devices was mounted on the same attachment systemas the cell culture plates. The modulatedsquare wave signal was amplified by a T.AMP E800audio amplifier to produce the desired g-force. Peak acceleration of3gwas achieved by adjusting the amplitude of theoutput signal, corresponding to the vertical displacement.
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