After incubation, the antibiotic extends to the agar beyond the zone of inhibition, but in lower concentrations, indicating that the microorganism can grow in small concentrations of the tested drug.
It is one of the simplest, most reliable and most used susceptibility methods in microbiology laboratories. Its basic principle is the diffusion of the antimicrobial on the surface of the agar, from a disc impregnated with the same antimicrobial.
The agar disk diffusion test was described in 1966 by Bauer and Kirby. It is one of the simplest and most reliable sensitivity methods. It is performed by dispensing the antimicrobial discs onto the agar plate after applying the bacterial inoculum with approximately 1 to 2 x 10 CFU/mL.
With this information, we can conclude that The purpose of the disk diffusion method is to assess the sensitivity or resistance of a microorganism to a particular antimicrobial agent.
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