The role of the dideoxy CTP is to occasionally terminate enzymatic synthesis of DNA where Gs occurs in the template stands. Sanger sequencing is also known as chain termination method in which determining the nucleotide sequence of DNA.
The method of DNA sequencing was developed by the Laureate Frederick Sanger and his colleagues in 1977. Dideoxynucleotides are used as terminate growing DNA chains and create the subsets of truncated fragments in a sequencing reaction.
The Sanger technique uses dideoxyneucleotides to typical deoxynucleotides in the reaction. ddNTP is terminated finally of that DNA stand because ddNTPs lacks 3' - OH group which required for phosphodiesterase bond formation between nucleotide.
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