Tris(hydroxymethyl)aminomethane [(HOCH₂)₃CNH₂], known as TRIS, is a weak base used in biochemical experiments to make buffer solutions in the pH range of 7 to 9. A certain TRIS buffer has a pH of 8.10 at 25°C and a pH of 7.80 at 37°C. Why does the pH change with temperature?

Respuesta :

when the Ka value changes, the pH value also changes.

We must keep in mind that every chemical reaction has an equilibrium constant K, and that K fluctuates as the temperature changes, in order to answer this problem.

If an exothermic reaction occurs,:

K value drops as temperature rises.

K value rises as temperature drops If the reaction is endothermic, the temperature rises and the K value rises.

K value drops as temperature rises

Since the Henderson-Hasselbalch equation includes the Ka value when there is a buffer, we know that when the Ka value changes, the pH value also changes:

[tex]PH= PKa+log \frac{A}{HA}[/tex]

What makes pH buffering crucial?

Small additions of acid or base can be neutralized by it, keeping the pH of the solution largely constant. For procedures and/or reactions that call for particular and stable pH ranges, this is significant.

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