what is the result of applying too much sample to the tlc plate? select one: the sample leeches out into the elution solvent reduced separation between the spots some of the sample moves down instead of up the spot does not leave the origin the sample moves past the solvent front

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21-10-2022
Chemistry

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what is the result of applying too much sample to the tlc plate? select one: the sample leeches out into the elution solvent reduced separation between the spots some of the sample moves down instead of up the spot does not leave the origin the sample moves past the solvent front

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lukanov1901 lukanov1901 · Answer 1 · 2022-11-01T17:31:08+01:00

Applying too much sample to the TLC (thin layer chromatography) plate results in reduced separation between the spots.

When performing thin layer chromatography it is necessary to not apply too much of the sample onto the plate. Once the elution solvent starts climbing up the plate, all the components of the sample will be separated along the plate according to the intensity of their interactions with the plate and the solvent (quantified as the Rf value - retention factor).

In a sample that is too concentrated or too much of it has been applied, the separation will be negatively affected because the huge spots originating from the sample components will overlap, making Rf determination difficult, if not impossible.

You can learn more about thin layer chromatography here:

brainly.com/question/13483325

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