you transform bacteria with a plasmid carrying the ampicillin-resistance gene ampr. how would you determine which bacteria took up the plasmid?

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21-11-2022
Biology

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you transform bacteria with a plasmid carrying the ampicillin-resistance gene ampr. how would you determine which bacteria took up the plasmid?

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abhinavjh321 abhinavjh321 · Answer 1 · 2022-11-23T18:02:36+01:00

The presence of ampicillin would permit the growth of bacteria that carry the plasmid.

There won't be any bacterial growth on the LB/Amp plate if the bacterium lacks the plasmid because the plasmid is required for the bacterium to grow in the presence of the antibiotic ampicillin.Only recombinant plasmid-containing cells will be white in the white colonies.

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