While ATP synthesis will decline, oxygen usage won't change.
Without influencing the respiratory chain or ATP synthase (H(+)-ATPase), uncouplers of oxidative phosphorylation in mitochondria prevent the coupling between the electron transport and phosphorylation processes. This inhibits ATP generation.
particle gradient , the inner mitochondrial membrane's electron transport chain receives electrons from NADH and FADH2, which causes an H+ accumulation in the inner membrane space. The direct energy source for ATP synthesis is this proton gradient (gradient of H+) flowing via the membrane enzyme complex ATP synthetase.
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