RNA polymerase is not used in PCR.
Two brief DNA sequences called primers are designed to anneal to the start and finish of the DNA target. Following that, the target DNA template is placed in a tube containing primers, free nucleotides, and the DNA polymerase enzyme. After that, the mixture is put into a PCR machine to carry out PCR.
Taq polymerase, primers, template DNA, and nucleotides are the main components of a PCR reaction (DNA building blocks). DNA can be created by assembling the components in a tube with the cofactors the enzyme requires and putting them through repeated cycles of heating and cooling.
Denaturation, annealing, and extension are the first three PCR steps, and subsequent cycling results in an exponential amplification of the target DNA.
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