A mutagen is tested to see if it can create colonies on a basic medium devoid of histidine, beginning with a hisG mutant strain of bacteria. This test is known as the basic Ames test for mutagenesis (option - d).
A thin layer of histidine is added to the agar plate with the bacteria. This tiny amount of histidine in the growth medium enables the bacteria to grow initially and give themselves the chance to mutate.
A popular technique is the Ames test, which uses bacteria to determine whether a specific chemical can result in DNA mutations in the test organism. To evaluate the mutagenic potential of chemical compounds, a biological assay is officially used.
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