ACh is released into the synaptic cleft in response to an action potential in the motor neuron. Gradient potentials are started when ACh binds to sarcolemma receptors.
The ryanodine receptors (RyRs) in the SR also open and release calcium as a result of the action potential's invasion of the T-tubules, which opens the L-type calcium channels and stimulates contraction.\
Sodium ions within a muscle cell start the action potential. When a muscle contracts, calcium ions are released into the muscle cell, strengthening the muscle. This occurs after the cell becomes positive, starting the action potential.
ACh is released into the synaptic cleft in response to a motor neuron action potential.
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