In glycolysis, hexokinase dephosphorylation prevents the transport of glucose back out of the cell.
With the help of ATP, hexokinase dephosphorylates glucose, resulting in a nonpolar glucose molecule that cannot pass through the hydrophilic region of the plasma membrane.
When glucose is phosphorylated by hexokinase utilising ADP, a polar glucose molecule is produced that cannot pass through the hydrophobic core of the plasma membrane.
The nonpolar glucose molecule produced by hexokinase's dephosphorylation of glucose using ADP is unable to pass the hydrophilic region of the plasma membrane.
Using ATP, hexokinase converts glucose to a polar form that cannot pass through the hydrophobic core of the plasma membrane.
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