Answer: A dichotomous key is typically used by checking observable characteristics of the organism through a sequence of two-part steps until an identification is listed in the key.
So, the correct option is:
Observable characteristics of the organism are checked through a sequence of two-part steps until an identification is listed in the key.
Explanation: In a dichotomous key, users are presented with a series of choices or questions about the characteristics of the organism being identified. Based on the characteristics observed, the user selects the option that best matches the organism. This process continues through a series of paired choices until a specific identification is reached.