The largest effect on an enzyme, A frameshift mutation near the end of a gene . Therefore, A frameshift mutation occurs when nucleotides are either inserted or deleted from a gene, causing a shift in the reading frame of the mRNA during translation.
This alteration disrupts the correct grouping of codons into amino acids, leading to a completely different sequence of amino acids and a likely nonfunctional or severely impaired protein.
In the case of a frameshift mutation near the end of a gene, the impact on the protein is substantial.
While a deletion of three nucleotides or an insertion of three nucleotides can cause a shift in the reading frame, the effects may be less severe if they occur toward the end of the gene.
However, any frameshift mutation has the potential to introduce premature stop codons, altering the normal termination of translation and resulting in a truncated and often nonfunctional protein.
This can lead to a loss of enzymatic activity or disruption of the protein's structure and function, making frameshift mutations particularly impactful on the resulting enzyme.
