In the reaction catalyzed by the pyruvate dehydrogenase complex, the two carbons constituting the acetyl group are oxidized by NAD+ while attached to the lipoamide.
A multienzyme complex called pyruvate dehydrogenase (PDH) catalyzes the oxidative conversion of pyruvate into acetyl coenzyme A (CoA), carbon dioxide (CO2), and reducing equivalents in the form of NADH.
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