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The more the number of theoretical plates, the more easier the separation of a mixture of two liquids.

A measure of column efficiency is the theoretical plates number (N). It describes the number of plates as defined by plate theory and can be used to calculate column efficiency using a computation where the sharper the peaks, the higher the theoretical plate number. The more theoretical plates there are, the more ideal equilibrium stages there are in the system, and the more effective the separation is.

From all of these factors, it is obvious that more theoretical plates suggest greater separation quality since more equilibriums between stationary and mobile phases can be reached. This can be understood by using the example of a set of stairs. The length of each step grows as the number of stairs decreases.

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